Composite

Part:BBa_K3766105:Design

Designed by: Kamyar Keshavarz-Farajkhah   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


pBad evoAPOBEC1-BE4max T7RNAP CGG-R12-KIRV


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
    Illegal EcoRI site found at 1580
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1904
    Illegal PstI site found at 1964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal EcoRI site found at 1580
    Illegal NheI site found at 1205
    Illegal PstI site found at 1904
    Illegal PstI site found at 1964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal EcoRI site found at 1580
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
    Illegal EcoRI site found at 1580
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1904
    Illegal PstI site found at 1964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
    Illegal EcoRI site found at 1580
    Illegal XbaI site found at 1218
    Illegal PstI site found at 1904
    Illegal PstI site found at 1964
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1259
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1952
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

to do


Source

DNA synthesis, PCR and assembly

References